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        <title>Assay Design</title>
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        <h1 align="center">Assay Design</h1>
		<p align="left"><b>Limitations of large-scale applications</b></p>
		<p>-a universal two temperature cycling regime: reasoning and utility</p>
		<p>-key issue for large-scale applications is the inability to conduct 
		optimization for each individual primer pair, </p>
		<p>-necessity for a universal cycling regime</p>
		<p>-</p>
		<p><b>Two temperature cycling regime with a </b></p>
		<p><b>Utility of a high annealing and elongation temperature </b></p>
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